Purification and characterisation of an antioxidative peptide from enzymatic hydrolysates of duck processing by-products |
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Authors: | Seung-Jae Lee Eun-Kyung Kim Jin-Woo Hwang Hyun-Jung Oh Sun-Hee Cheong Sang-Ho Moon Byong-Tae Jeon Sang Moo Lee Pyo-Jam Park |
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Affiliation: | 1. Department of Biotechnology, Konkuk University, Chungju 380-701, Republic of Korea;2. Korea Nokyong Research Center, Konkuk University, Chungju 380-701, Republic of Korea;3. Department of Animal Science, Kyungbuk University, Sangju 742-711, Republic of Korea |
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Abstract: | Duck processing by-products were hydrolysed using eight proteases to produce an antioxidative peptide. Of the various hydrolysates produced, the pepsin extract exhibited the highest hydroxyl radical scavenging activity. The derived peptide was purified using high-performance liquid chromatography (HPLC) to identify any potent radical scavenging activity. The sequence of the antioxidative peptide obtained was identified as Asp-Val-Cys-Gly-Arg-Asp-Val-Asn-Gly-Tyr, with a molecular weight of 1096 Da. The IC50 value of purified peptide for hydroxyl radical scavenging activity was 75 μg/ml as the measurement by an electron spin resonance (ESR) spectrometer. In addition, the purified peptide exhibited a protective effect on H2O2-induced DNA damage. These results indicate that the purified peptide possesses a potent antioxidative activity and protective effect of DNA against H2O2-induced oxidative damage. |
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Keywords: | Antioxidative peptide Hydroxyl radical Duck processing by-product Enzymatic hydrolysis |
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