高效液相色谱法测定水产品中甲基睾丸酮残留量的优化研究

优化测定水产品中甲基睾丸酮残留量的高效液相色谱法。样品经乙醚超声波辅助提取、石油醚净化，以ODS-C18 柱，77% 甲醇溶液洗脱分离，紫外检测器检测，外标法定量。结果表明，用本法测定罗非鱼(T.niloticus)、对虾(L. vannamei)和鳗鲡(A. anguilla)3 种水产品中的甲基睾丸酮的残留量，其回收率不低于83%，相对标准偏差不高于6.48%，定量限为8μg/kg。该法准确可行、前处理简便、适用于批量样品的测定。

HPLC Determination of 17α-Methyltestosterone Residue in Aquatic Products

A simple high-performance liquid homographic (HPLC) method was presented for the determination of 17α- methyltestosterone (17α-MeT) residue in aquatic products. Samples were extracted using ether with the assistance of ultrasounic and cleaned up using petroleum ether. The chromatographic separation was achieved on ODS-C18 column using 77% aqueous methanol solution as an eluant. 17α-MeT was detected using a UV detector and quantified by external standard method. The method revealed spike recoveries for 17α-methyltestosterone in the muscles of T. niloticus, L. vannamei and A. anguilla of not less than 83%, with relative standard deviations of not more than 6.48% and a limit of quantification of 8 μg/kg. The method, thanks to its simplicity and accuracy, is most suitable for the determination of 17α-MeT residue in large quantities of samples.