基于静电纺丝技术的PLGA载药纳米纤维膜的制备工艺

同轴静电纺丝法制备的聚乳酸-乙醇酸(PLGA)纳米纤维具有良好的生物相容性和生物可降解性, 加之其高孔隙率和高透氧率, 使其能成为优良的药物载体。本文初步摸索了PLGA的同轴静电纺丝的工艺条件, 并通过同轴静电纺丝法制备了PLGA载氟比洛芬酯(FA)的纳米纤维膜, 应用扫描电子显微镜、红外光谱分析观察纤维的表观形貌并确定其微观结构。重点探究了不同溶剂配比的混合溶剂对载药纤维膜药物释放性能影响。研究结果表明在U⁺为+15.00kV, U^-为-2.50kV, 接受距离为15cm, 壳层推进速度为0.4mm/min, 芯层推进速度为0.1mm/min进行静电纺丝时, 所制备的PLGA(壳)/PVP+FA(核)复合载药纤维膜壳核结构良好, 且成功载了约0.5%的FA。当改变壳层混合溶剂(DCM和DMF)和芯层混合溶剂(无水乙醇和DMF)体积比时, 纤维直径会随着DMF的减少而增大。

Preparation of drug-loaded polylactic acid-based fibers membranes based on electrospinning technology

PLGA poly(1actic-co-glycolic acid)] coaxial electrospinning nanofiber is a biodegradable material with good biocompatibility. In addition, its high porosity and high oxygen permeability would also favor PLGA to become a good pharmaceutical carrier. The technological conditions of electrospinning were studied and flurbiprofen axetil (FA)-loaded PLGA nanofibers were prepared by coaxial electrospinning. The structure and morphology of the nanofiber were characterized by infrared spectrometer (IR) and scanning electron microscope (SEM), respectively. The influence of solvent's proportion on nanofibers'drug release properties was investigated. When positive voltage, negative voltage, receive distance, advancing speed of the shell as well as the core is+15.00kV, -2.50kV, 15cm, 0.4mm/min, 0.1mm/min, respectively. The prepared PLGA/PVP FA-loaded electrospun fibers had a good core-shell structure, and carried successfully about 0.5% FA. Besides, when volume ratio of the shell's mixed solvent (DCM and DMF) and the core's mixed solvent (anhydrous ethanol and DMF) changed, fiber diameter would increase with the decrease of DMF.