Upregulation of HLA class-I gene transcription in K562 cells by analogs of splenopentin (SP-5) |
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| Authors: | M Chatterjee-Kishore S Agrawal VK Singh KB Mathur A Haq SS Agarwal |
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| Affiliation: | Babes-Bolyai University, Analytical Chemistry Department, Cluj-Napoca, Romania. |
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| Abstract: | The carotenoid composition of fruits of Rosa canina (Rosaceae) was determined comparatively by thin-layer chromatography (TLC) and high-performance liquid chromatography (HPLC) in total extracts and in three different fractions derived from previous separation of the total fruit extract on alumina columns. Both chromatographic analyses revealed as major carotenoids: beta-carotene, lycopene, beta-chryptoxanthin, rubixanthin, zeaxanthin and lutein. The distribution of these compounds was reproducible by TLC and by HPLC. The I-III fractions eluted successively from alumina columns by increasing the polarity of the solvents were analysed also by TLC and HPLC. In all situations, carotenoids were better separated and identified by gradient HPLC systems than by isocratic HPLC or TLC. |
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