| 重组鲈鱼生长激素的酶联免疫测定方法 |
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| 引用本文： | 刘小青,陈荣忠,杨丰. 重组鲈鱼生长激素的酶联免疫测定方法[J]. 高技术通讯, 2002, 12(2): 40-42 |
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| 作者姓名： | 刘小青 陈荣忠 杨丰 |
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| 作者单位： | 1. 厦门大学生命科学院,厦门,361005;国家海洋局第三海洋研究所,厦门,361005
2. 国家海洋局第三海洋研究所,厦门,361005 |
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| 基金项目： | 国家九五攻关课题 ( 96 C0 1 0 5 0 4)和福建省科技 ( 99 Z 9)资助项目 |
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| 摘 要： | 建立了重组鲈鱼生长激素（jspGH0的间接ELISA，以jspGH的小鼠多克隆抗体作为一抗，HRP羊抗鼠IgG作为二抗，在抗体及酶标二抗的稀释液中加入4％PEG，从而提高检测灵敏度至40ng/ml，抗原浓度在40－1000ng/ml范围内与OD450nm值成线性关系。此法较简便，灵敏，可用于检测基因工程菌发酵液中的jspGH含量。
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| 关 键 词： | 酶联免疫测定方法 重组鲈鱼生长激素 jspGH 间接ELISA 发酵液 基因工程 |
An Indirect Enzyme-linked Immunosorbent Assay for the Recombinant Japanese Set Perch Growth Hormone |
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| Abstract: | An indirect enzyme linked immunoassay (ELISA) for recombinant Japanese sea perch growth hormone (jspGH) was established by adding 4%PEG in the first and the second antibody solution. The sensitivity of ELISA is about 40ng/ml. A linear response between the concentration of jspGH and OD 450nm is obtained in the range of 40 1000ng/ml. When the yeast cell line GS115 not containing jspGH was set as the control, the concerntration of jspGH could be calculated from the curve. The method is simple and rapid, and can be used to detect the level of jspGH in fermentaton liquid. |
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| Keywords: | Recombinant japanese sea perch growth hormone Indirect ELISA Fermentation liquid |
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