DETECTION OF VIBRIO ALGINOLYTICUS AND VIBRIO PARAHAEMOLYTICUS IN SHELLFISH SAMPLES USING COLLAGENASE-TARGETED MULTIPLEX-PCR |
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| Authors: | ANGELA DI PINTO GIUSEPPINA CICCARESE MARIA FONTANAROSA VALENTINA TERIO GIUSEPPINA TANTILLO |
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| Affiliation: | Dipartimento di Sanitàe Benessere degli Animali Sezione di Ispezione degli Alimenti Facoltàdi Medicina Veterinaria Universitàdegli Studi di Bari Strada Prov.le per Casamassima km 3 –70010 Valenzano (Bari), Italy; Istituto Zooprofilattico Sperimentale della Puglia e della Basilicata S.S. 7 ter, km 59, Zona Industriale 73012 Campi Salentina (Lecce), Italy |
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| Abstract: | The increase of Vibrio infections especially associated with the consumption of contaminated seafood and fish underlines the necessity of an efficient monitoring system for Vibrio spp. supported by appropriate detection tools both in food products and in clinical samples. The aim of this study was the evaluation of the reliability of the collagenase‐targeted multiplex‐polymerase chain reaction (m‐PCR) for the detection of Vibrio alginolyticus, Vibrio cholerae and Vibrio parahaemolyticus in shellfish samples enriched in alkaline peptone water (APW). The coupling of a pre‐enrichment phase of the samples with the specificity of the PCR‐based assay was applied successfully to the detection of V. alginolyticus and V. parahaemolyticus, demonstrating that collagenase‐target m‐PCR may be used as a valid molecular target to discriminate the three Vibrio species. |
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