A micro‐method for lipoprotein cholesterol profiles: Impact of CETP in KKAy mice |
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Authors: | Pe¯teris Alberts Gunnel Klingstrm Vibeke Arrhenius‐Nyberg Catarina Larsson Kjell S Sakariassen |
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Affiliation: | Pe¯teris Alberts,Gunnel Klingström,Vibeke Arrhenius‐Nyberg,Catarina Larsson,Kjell S. Sakariassen |
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Abstract: | The aim of the present study was to assess cholesterol‐containing lipoprotein profiles in minute serum samples. The lipoprotein profiles of KKAy and transgenic KKAy‐CETP mice and of other species were determined. The transgenic KKAy‐CETP mice express the simian enzyme cholesteryl ester transfer protein (CETP). The serum profile of the cholesterol‐containing high‐density (HDL), low‐density (LDL) and very‐low‐density lipoproteins (VLDL) was monitored on a Superose 6 column using fast protein liquid chromatography. Serum from several mouse and rat strains, rabbit, hamster, pig and man was included for comparative and method validation purposes. The chromatograms showed that the transgenic KKAy‐CETP mice had significantly decreased relative levels of HDL vs. VLDL and LDL cholesterol (p <0.001). Introduction of the CETP gene shifted the serum profile of the cholesterol‐containing lipoproteins of the KKAy‐CETP mice closer to the human profile in a dose‐dependent manner, thus making these mice an interesting model for man. The described lipoprotein separation technology offers promising and reliable opportunities for studies of blood lipoprotein profiles with minute serum samples, in both animals and man. |
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Keywords: | HDL CETP diabetes metabolic disease obesity |
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