A computer model for the analysis of DNA replication intermediates by two-dimensional agarose gel electrophoresis |
| |
Authors: | E Viguera A Rodríguez P Hernández DB Krimer O Trellez JB Schvartzman |
| |
Affiliation: | Departamento de Biología Celular y del Desarrollo, CIB (CSIC), Velázquez 144, 28006, Madrid, Spain. |
| |
Abstract: | We present a computer model to predict the patterns expected for the replication intermediates (RIs) of DNA fragments analyzed by neutral/neutral two-dimensional (2D) agarose gel electrophoresis. The model relies on the mode of replication (uni- or bi-directional), the electrophoretic mobility of linear DNA fragments and the retardation caused by the three-dimensional shape of non-linear molecules. The utility of this model is demonstrated with two examples: replication analysis of the plasmids pBR322 and pHH5.8 in Escherichia coli after digestions with EcoRI and HindIII, respectively. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|