Deciphering Specificity Determinants for FR900359‐Derived Gqα Inhibitors Based on Computational and Structure–Activity Studies |
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Authors: | Raphael Reher Dr Toni Kühl Suvi Annala Tobias Benkel Desireé Kaufmann Britta Nubbemeyer Justin Patrick Odhiambo Dr Pascal Heimer Charlotte Anneke Bäuml Dr Stefan Kehraus Dr Max Crüsemann Prof?Dr Evi Kostenis Dr Daniel Tietze Prof?Dr Gabriele M König Prof?Dr Diana Imhof |
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Affiliation: | 1. Institute of Pharmaceutical Biology, University of Bonn, Bonn, Germany;2. Pharmaceutical Biochemistry and Bioanalytics, Pharmaceutical Institute, University of Bonn, Bonn, Germany;3. Molecular, Cellular and Pharmacobiology Section, Institute of Pharmaceutical Biology, University of Bonn, Bonn, Germany;4. Eduard Zintl Institute for Inorganic and Physical Chemistry, Technische Universit?t Darmstadt, Darmstadt, Germany |
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Abstract: | Direct targeting of intracellular Gα subunits of G protein‐coupled receptors by chemical tools is a challenging task in current pharmacological studies and in the development of novel therapeutic approaches. In this study we analyzed novel FR900359‐based analogs from natural sources, synthetic cyclic peptides, as well as all so‐far known Gqα inhibitors in a comprehensive study to devise a strategy for the elucidation of characteristics that determine interactions with and inhibition of Gq in the specific FR/YM‐binding pocket. Using 2D NMR spectroscopy and molecular docking we identified unique features in the macrocyclic structures responsible for binding to the target protein correlating with inhibitory activity. While all novel compounds were devoid of effects on Gi and Gs proteins, no inhibitor surpassed the biological activity of FR. This raises the question of whether depsipeptides such as FR already represent valuable chemical tools for specific inhibition of Gq and, at the same time, are suitable natural lead structures for the development of novel compounds to target Gα subunits other than Gq. |
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Keywords: | depsipeptides Gα protein inhibitors Gα proteins macrocyclic peptides NMR spectroscopy |
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