Fully Automated Production and Characterization of 64Cu and Proof‐of‐Principle Small‐Animal PET Imaging Using 64Cu‐Labelled CA XII Targeting 6A10 Fab |
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| Authors: | Luise Fiedler Dr. Markus Kellner Rosel Oos PD Dr. Guido Böning Prof. Sibylle Ziegler Prof. Peter Bartenstein Prof. Reinhard Zeidler Dr. Franz Josef Gildehaus Dr. Simon Lindner |
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| Affiliation: | 1. Department of Nuclear Medicine, University Hospital, LMU Munich, Munich, Germany;2. Helmholtz-Zentrum München, German Research Center for Environmental Health, Research Group Gene Vectors, Munich, Germany;3. Department of Otorhinolaryngology, University Hospital, LMU Munich, Munich, Germany |
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| Abstract: | 64Cu is a cyclotron‐produced radionuclide which offers, thanks to its characteristic decay scheme, the possibility of combining positron emission tomography (PET) investigations with radiotherapy. We evaluated the Alceo system from Comecer SpA to automatically produce 64Cu for radiolabelling purposes. We established a 64Cu production routine with high yields and radionuclide purity in combination with excellent operator radiation protection. The carbonic anhydrase XII targeting 6A10 antibody Fab fragment was successfully radiolabelled with the produced 64Cu, and proof‐of‐principle small‐animal PET experiments on mice bearing glioma xenografts were performed. We obtained a high tumor‐to‐contralateral muscle ratio, which encourages further in vivo investigations of the radioconjugate regarding a possible application in diagnostic tumor imaging. |
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| Keywords: | 64Cu 6A10 Fab Comecer Alceo positron emission tomography solid targets |
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