| 亚甲基蓝为介体的丝网印刷双酶修饰电极测定葡萄糖 |
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| 引用本文： | 徐肖邢.亚甲基蓝为介体的丝网印刷双酶修饰电极测定葡萄糖[J].食品科学,2007,28(4):249-251. |
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| 作者姓名： | 徐肖邢 |
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| 作者单位： | 常熟理工学院化学与材料工程系 江苏 常熟 215500； |
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| 基金项目： | 江苏省教育厅自然科学基金 |
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| 摘 要： | 以亚甲基蓝作为电子媒介,通过壳聚糖固定葡萄糖氧化酶和辣根过氧化酶在丝网印刷电极上,制备成葡萄糖生物传感器。循环伏安法和恒电位法用于研究修饰电极的电化学特性,在优化的实验条件下,获得传感器对葡萄糖响应的线性范围为8．0×10-5～3．5×10-3mol/L。此方法测定葡萄糖抗干扰能力强、重现性好、准确度高,电极制作简单,使用方便。
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| 关 键 词： | 葡萄糖 葡萄糖氧化酶 辣根过氧化物酶 丝网印刷电极 生物传感器 |
| 文章编号： | 1002-6630（2007）04-0249-03 |
| 修稿时间： | 2006-03-30 |
Determination of Glucose by Bienzyme Modified Screen-printed Electrode Based on Methylene Blue |
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| XU Xiao-xing.Determination of Glucose by Bienzyme Modified Screen-printed Electrode Based on Methylene Blue[J].Food Science,2007,28(4):249-251. |
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| Authors: | XU Xiao-xing |
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| Affiliation: | Department of Chemistry and Materials Engineering,Changshu Institute of Technology,Changshu 215500,China |
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| Abstract: | A new glucose biosensor has been made by chitosan to immobilize glucose oxidase and horseradish peroxidase on the screen-printed electrode surface.The system is based on methylene blue as an electron shuttle between peroxidase and electrode.Cyclic voltammetry and current-time curve were employed to investigate the electrochemical characteristics of the glucose biosensor.Under optimal conditions,the range of the sensor response to glucose is from 8.0×10-5mol/L to 3.5×10-3mol/L.This method of glucose detection displayed better anti-jamming capacity and high reproducibility.The screen-printed electrode is simple to prepare and easy to use. |
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| Keywords: | glucose glucose oxidase horseradish peroxidase screen-printed electrode biosensor |
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