果蔬生防菌葡萄有孢汉逊酵母CM2糖蜜培养基的优化

以葡萄有孢汉逊酵母CM2为研究对象，细胞生物量为测定指标，利用甘蔗糖蜜作为发酵原料，对糖蜜培养基的优化进行较为全面的探讨。采用煮沸法、磷酸法、硫酸法、活性炭法、亚铁氰化钾法对糖蜜进行预处理，结果表明硫酸处理法优于其他处理方法。通过单因素试验研究糖蜜质量浓度、氮源及其添加量、初始pH值、磷酸盐、硫酸镁、氯化钠等培养基组分对CM2生长的影响；采用Plackett-Burman试验设计、最陡爬坡试验和中心组合试验设计对培养基组分进行优化，得到优化培养基组分：预处理后糖蜜78.9 g/L、酵母粉2.2 g/L、初始pH 6.0。通过验证实验获知与PDB培养基相比，优化后糖蜜培养基使CM2生物量提高了83.1%。

Optimization of Molasses Medium Composition for Hanseniaspora uvarum CM2 as a Biocontrol Yeast

The composition of a sugarcane molasses-based medium used to culture Hanseniaspora uvarum CM2 was
optimized for enhanced biomass production. The molasses was pretreated by boiling or adding phosphoric acid, sulfuric
acid, potassium ferrocyanide and activated carbon, respectively. Comparing their impacts on the growth of the antagonistic
yeast CM2, sulfuric acid addition was considered the method of choice. Molasses concentration, the type and concentration
of nitrogen source, initial pH, phosphate, magnesium sulfate, and sodium chloride were studied for their influence on the
biomass production of CM2 by single factor design. Plackett-Burman design and response surface methodology were used
to optimize the medium components as pretreated molasses 78.9 g/L, yeast powder 2.2 g/L and initial pH 6.0. The optimal
medium could result in an 83.1% enhancement in the biomass production of CM2.