Human Mesenchymal Stem Cell-Derived Extracellular Vesicles Promote Neural Differentiation of Neural Progenitor Cells |
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| Authors: | So-Yeon Park Da-Seul Kim Hyun-Mun Kim Jun-Kyu Lee Dong-Youn Hwang Tae-Hyung Kim Seungkwon You Dong Keun Han |
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| Affiliation: | 1.Department of Biomedical Science, CHA University, 335 Pangyo-ro, Bundang-gu, Seongnam-si 13488, Korea; (S.-Y.P.); (D.-S.K.); (H.-M.K.); (J.-K.L.); (D.-Y.H.);2.Division of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul 02841, Korea;3.School of Integrative Engineering, Chung-Ang University, 84 Heukseok-ro, Seoul 06974, Korea; |
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| Abstract: | Mesenchymal stem cells (MSCs) have been adopted in various preclinical and clinical studies because of their multipotency and low immunogenicity. However, numerous obstacles relating to safety issues remain. Therefore, MSC-derived extracellular vesicles (EVs) have been recently employed. EVs are nano-sized endoplasmic reticulum particles generated and released in cells that have similar biological functions to their origin cells. EVs act as cargo for bioactive molecules such as proteins and genetic materials and facilitate tissue regeneration. EVs obtained from adipose-derived MSC (ADMSC) also have neuroprotective and neurogenesis effects. On the basis of the versatile effects of EVs, we aimed to enhance the neural differentiation ability of ADMSC-derived EVs by elucidating the neurogenic-differentiation process. ADMSC-derived EVs isolated from neurogenesis conditioned media (differentiated EVs, dEVs) increased neurogenic ability by altering innate microRNA expression and cytokine composition. Consequently, dEVs promoted neuronal differentiation of neural progenitor cells in vitro, suggesting that dEVs are a prospective candidate for EV-based neurological disorder regeneration therapy. |
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| Keywords: | extracellular vesicle (EV) mesenchymal stem cell neural progenitor cell neurogenesis microRNA cytokine |
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