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QTL Mapping and Candidate Gene Analysis for Seed Germination Response to Low Temperature in Rice
Authors:Nari Kim  Rahmatullah Jan  Jae-Ryoung Park  Saleem Asif  Dan-Dan Zhao  Eun-Gyeong Kim  Yoon-Hee Jang  Gyu-Hyeon Eom  Gang-Seob Lee  Kyung-Min Kim
Abstract:Low temperature is a serious threat to the seed emergence of rice, which has become one of the main limiting factors affecting rice production in the world. It is of great significance to find the candidate genes controlling low-temperature tolerance during seed germination and study their functions for breeding new rice cultivars with immense low-temperature tolerance during seed germination. In the current experiment, 120 lines of the Cheongcheong Nagdong Double Haploid (CNDH) population were used for quantitative trait locus (QTL) analysis of low-temperature germinability. The results showed a significant difference in germination under low different temperature (LDT) (15 °C, 20 °C) conditions. In total, four QTLs were detected on chromosome 3, 6, and 8. A total of 41 genes were identified from all the four QTLs, among them, 25 genes were selected by gene function annotation and further screened through quantitative real-time polymerase chain reaction (qRT-PCR). Based on gene function annotation and level of expression under low-temperature, our study suggested the OsGPq3 gene as a candidate gene controlling viviparous germination, ABA and GA signaling under low-temperature. This study will provide a theoretical basis for marker-assisted breeding and lay the basis for further mining molecular mechanisms of low-temperature germination tolerance in rice.
Keywords:low-temperature   seed germination   Cheongcheong Nagdong double haploid   quantitative trait locus   viviparous germination   marker-assisted breeding
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