A Novel Artificial Hemoglobin Carrier Based on Heulandite-Calcium Mesoporous Aluminosilicate Particles |
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| Authors: | Dino Jordanoski,Damjana Drobne,Než a Repar,Iztok Dogsa,Polona Mrak,Romana Cerc-Koroš ec,Andrijana Sever Š kapin,Peter Nadrah,Natasa Poklar Ulrih |
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| Affiliation: | 1.Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, 1000 Ljubljana, Slovenia; (D.J.); (D.D.); (N.R.); (I.D.); (P.M.);2.Faculty of Chemistry and Chemical Technology, University of Ljubljana, Večna Pot, 1000 Ljubljana, Slovenia;3.Slovenian National Bulding and Civil Engineering Institute, Dimičeva Ulica 12, 1000 Ljubljana, Slovenia; (A.S.Š.); (P.N.) |
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| Abstract: | Tetraethyl-orthosilicate (TEOS)-based nanoparticles are most extensively used as a silica-based hemoglobin carrier system. However, TEOS-based nanoparticles induce adverse effects on the hemoglobin structure. Therefore, a heulandite-calcium-based carrier was investigated as a novel silica-based hemoglobin carrier system. The heulandite-calcium mesoporous aluminosilicate particles (MSPs) were fabricated by a patented tribo-mechanical activation process, according to the manufacturer, and its structure was assessed by X-ray diffraction analysis. Upon hemoglobin encapsulation, alternation in the secondary and tertiary structure was observed. The hemoglobin-particle interactions do not cause heme degradation or decreased activity. Once encapsulated inside the particle pores, the hemoglobin shows increased thermal stability, and higher loading capacity per gram of particles (by a factor of >1.4) when compared to TEOS-based nanoparticles. Futhermore, we introduced a PEGlyted lipid bilayer which significantly decreases the premature hemoglobin release and increases the colloidal stability. The newly developed hemoglobin carrier shows no cytotoxicity to human umbilical vein endothelial cells (HUVEC). |
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| Keywords: | silica particles hemoglobin carrier encapsulation mesoporous spectroscopy |
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