State-of-the-Art on Wound Vitality Evaluation: A Systematic Review |
| |
| Authors: | Aniello Maiese Alice Chiara Manetti Naomi Iacoponi Eleonora Mezzetti Emanuela Turillazzi Marco Di Paolo Raffaele La Russa Paola Frati Vittorio Fineschi |
| |
| Affiliation: | 1.Department of Surgical, Medical and Molecular Pathology and Critical Care Medicine, Institute of Legal Medicine, University of Pisa, 56126 Pisa, Italy; (A.M.); (A.C.M.); (N.I.); (E.M.); (E.T.); (M.D.P.);2.Department of Clinical and Experimental Medicine, University of Foggia, 71122 Foggia, Italy;3.Department of Anatomical, Histological, Forensic and Orthopedic Sciences, Institute of Legal Medicine, Sapienza University of Rome, Viale Regina Elena 336, 00161 Rome, Italy; |
| |
| Abstract: | The vitality demonstration refers to determining if an injury has been caused ante- or post-mortem, while wound age means to evaluate how long a subject has survived after the infliction of an injury. Histology alone is not enough to prove the vitality of a lesion. Recently, immunohistochemistry, biochemistry, and molecular biology have been introduced in the field of lesions vitality and age demonstration. The study was conducted according to the preferred reporting items for systematic review (PRISMA) protocol. The search terms were “wound”, “lesion”, “vitality”, “evaluation”, “immunohistochemistry”, “proteins”, “electrolytes”, “mRNAs”, and “miRNAs” in the title, abstract, and keywords. This evaluation left 137 scientific papers. This review aimed to collect all the knowledge on vital wound demonstration and provide a temporal distribution of the methods currently available, in order to determine the age of lesions, thus helping forensic pathologists in finding a way through the tangled jungle of wound vitality evaluation. |
| |
| Keywords: | vitality wound autopsy histology immunohistochemistry protein quantification ribonucleic acids |
|
|
