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The assessment of particle association and UV disinfection of wastewater using indigenous spore-forming bacteria |
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| Authors: | Li Dong Craik Stephen A Smith Daniel W Belosevic Miodrag |
| Affiliation: | a AECOM, #300, 340 Midpark Way SE, Calgary, Alberta T2X 1P1, Canada b EPCOR Water Services Inc., 10065 Jasper Avenue, Edmonton, Alberta T5J 3B1, Canada c Department of Civil and Environmental Engineering, University of Alberta, Edmonton, Alberta T6G 2W2, Canada d Department of Biological Sciences 13-103 Clinical Sciences Building, University of Alberta, Edmonton, Alberta T6G 2G3, Canada e Department of Public Health Sciences, 13-103 Clinical Sciences Building, University of Alberta, Edmonton, Alberta T6G 2G3, Canada |
| Abstract: | Studies have shown that association between particles and coliform bacteria in wastewater influence the inactivation of these microorganisms by ultraviolet (UV) irradiation. This research investigated the potential use of indigenous aerobic spore-forming (ASF) bacteria for studying the particle - microorganism interaction and its effect on UV disinfection of protozoan pathogens, such as Giardia spp. and Cryptosporidium spp., present in effluents from full-scale municipal wastewater treatment plants. The effect of particle - ASF association was determined by homogenizing wastewater effluent samples before and after exposure to controlled UV doses delivered by a bench-scale collimated beam apparatus. Particle association between Bacillus subtilis spores added to wastewater and wastewater particles was also assessed. The results indicate that spores are not significantly associated with wastewater particulate matter and particle association does not significantly affect the inactivation of indigenous spores present in wastewater by UV radiation in this study. |
| Keywords: | Ultraviolet Disinfection Particle association Wastewater Indigenous aerobic spore-forming bacteria Surrogate |
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