大孔树脂吸附分离长春花中的文多灵、长春质碱和长春碱

通过对11种国产大孔树脂对文多灵、长春质碱和长春碱的静态吸附容量和解吸率等指标的考察，筛选出AB-8大孔吸附树脂作为分离长春花生物碱的载体，其对文多灵、长春质碱和长春碱吸附量分别为365.8、254.2、24.8 mg·ml^-1。利用大孔吸附树脂吸附分离长春花3种生物碱的过程为：长春花原料用稀硫酸溶液提取，提取液用氨水调节pH值为8，然后采用AB-8大孔吸附树脂柱吸附，用20%乙醇洗涤除去强极性成分，在30℃下用pH=4的50%乙醇解吸，得单吲哚生物碱文多灵和长春质碱，再用90%乙醇解吸，得双吲哚生物碱长春碱。单吲哚生物碱纯度可达50%以上，长春碱纯度达60%以上。

Separation of vindoline,catharanthine and vinblastine from Catharanthus roseus (L.) G.Don with macroporous adsorption resin

Eleven different macroporous adsorption resins were studied with respect to the static adsorption capacity and desorption ratio of vinblastine, vindoline and catharanthine.The best resin was AB-8 and the adsorption capacities of vindoline, catharanthine and vinblastine were 365.8, 254.2 and 24.8 mg·ml^-1, respectively.The optimum parameters of separation process by AB-8 resin were as follows: extraction solvent was sulfate dilution; pH value of the extraction was adjusted to 8 by ammonia; elute solvents were 20% alcohol to remove polar components, 50% alcohol (pH=4) to obtain the mono-indole alkaloids (vindoline and catharanthine) at 30℃ and 90% alcohol to obtain the dimeric-indole alkaloid (vinblastine).The results showed that the content of mono-indole alkaloids was above 50% and the purity of vinblastine was above 60%.