Purification and partial sequence of proteins involved in the cholic acid transport into rat liver hepatocytes |
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Authors: | A Hoffmann S Müllner K Ziegler H Fasold |
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Affiliation: | Biozentrum Frankfurt, Abt. Biochemie, N 210/220, Germany. |
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Abstract: | Two proteins, in previous work labeled by affinity markers derived from taurocholic acid, were purified and partially sequenced. Antibodies were raised against purified proteins, and cross-reactions were carefully checked. The influence of these antibodies upon taurocholic acid import into vesicles from rat liver plasma membranes was measured, and showed a distinct inhibition of transport in the case of the 54 kD protein. |
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