| 生物合成共轭亚油酸菌种的筛选与鉴定 |
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| 引用本文： | 周凌华,张灏,陈卫,田丰伟. 生物合成共轭亚油酸菌种的筛选与鉴定[J]. 食品与生物技术学报, 2004, 23(5): 53-57 |
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| 作者姓名： | 周凌华 张灏 陈卫 田丰伟 |
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| 作者单位： | 江南大学,乳酸菌发酵技术与食品安全教育部重点实验室,江苏,无锡,214036 |
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| 摘 要： | 从传统泡菜和生牛乳中筛选出一株乳酸菌ZS2058能生物合成共轭亚油酸,经API系统鉴定为植物乳杆菌(Lactobacillusplantarum).该菌株在MRS培养基中将质量分数11.6%的亚油酸(1.024mg/mL)转化为共轭亚油酸,经气相色谱分析证实c9,t11 18∶2占75.9%,t10,c12 18∶2占24.1%.
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| 关 键 词： | 共轭亚油酸 乳酸菌 生物合成 鉴定 |
| 文章编号： | 1009-038X(2004)05-0053-05 |
| 修稿时间： | 2003-12-05 |
Screening and Identification of Lactic Acid Bacteria for Biosynthesis of Conjugated Linoleic Acid |
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| ZHOU Ling-hu,ZHANG Hao~,CHEN Wei,TIAN Feng-wei and Food Safety,Ministry of Education,Southern Yangtze University,Wuxi,China. Screening and Identification of Lactic Acid Bacteria for Biosynthesis of Conjugated Linoleic Acid[J]. Journal of Food Science and Biotechnology, 2004, 23(5): 53-57 |
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| Authors: | ZHOU Ling-hu ZHANG Hao~ CHEN Wei TIAN Feng-wei Food Safety Ministry of Education Southern Yangtze University Wuxi China |
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| Affiliation: | ZHOU Ling-hua,ZHANG Hao~*,CHEN Wei,TIAN Feng-wei and Food Safety,Ministry of Education,Southern Yangtze University,Wuxi 214036,China) |
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| Abstract: | A strain ZS2058 was selected from the strains separated from fermented vegetables and raw milk for biosynthesis of conjugated linoleic acid. The strain was identified as Lactobacillus plantarum by API system. The strain could tolerate at 11.6%(1.024 mg/mL) LA and converte LA to CLA in MRS broth. The cis-9, trans-11 and trans-10, cis-12 isomers detected by Gas Chromatography were 75.9% and 24.1 % of the CLA produced. |
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| Keywords: | conjugated linoleic acid lactic acid bacteria biosynthesis identification |
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