大孔树脂分离纯化花生壳水溶性膳食纤维工艺条件研究

选取8种大孔吸附树脂进行静态吸附性能研究,筛选出最有效D101树脂分离纯化花生壳水溶性膳食纤维。通过动态吸附性能研究,确定应用D101树脂分离纯化花生壳水溶性膳食纤维优化工艺条件;在上样流速2 ml/min、上样液质量浓度为1 mg/mL² mg/mL、上样液pH为10左右条件下吸附较强;在洗脱剂乙醇体积分数为70%、洗脱液流速为1 ml/min洗脱时洗脱效果最好。经紫外光谱扫描及SDF纯化物纯度测定结果显示,经纯化后花生壳水溶性膳食纤维纯度较高,可达96.4%;且蛋白含量较少,仅为0.23%。

Separation and purification of soluble dietary fiber from peanut null with macroporous resin

Eight kinds of macroporous resins adsorption property had been investigated in isolating and purifying soluble dietary fiber from peanut null.D101 type resin as the optimal absorbent material for soluble dietary fiber from peanut null was decided and its dynamic absorbing behavior was studied.The results showed that:D101 type resin possesses higher absorption and desorption capacity;the optimal technological conditions of adsorption velocity was 2 ml/min,solution concentration was 1 mg/mL to 2 mg/mL,solution pH was about 10,and the eluting solvent was 70% ethanol at 1 ml/min flow rate.UV spectral analysis and purity determination indicated the purified soluble dietary fiber from peanut null had high purity(96.4%)and contained little impurity(0.23%).