发酵液中纳他霉素含量快速检测方法研究

对紫外分光光度法和高效液相色谱法（HPLC）测定纳他霉素的各项参数进行了研究，结果表明：紫外分光光度法，确定甲醇＋1％冰乙酸（pH3．4）为提取剂，配比为1：9，303nm为检测波长，以纳他霉素标准溶液做标准曲线，求得线性回归方程为Y=0．1066X-0．0069，R^2=0．9995，纳他霉素回收率超过99．9314％。紫外分光光度法与高效液相色谱法两种检测方法结果基本一致，以高效液相色谱为基准，紫外分光光度法的相对误差为0．17％。最终确定高效液相色谱法作为检测发酵液中纳他霉素含量的方法。检测波长：303nm；流动相：甲醇：水：冰乙酸体积比为70：30：2；流速：1．00mL/min；检测器Waters2996二极管阵列检测器；线性回归方程为Y=12500X＋12600，R^2=0．9995，精密度试验结果：平均RSD为0．753％。当信噪比为3时的最小检测限为31ng。

THE RESEARCH OF RAPID DETERMINATION METHODS OF NATAMYCIN IN FERMENTATION SOLUTION

The ultraviolet spectrophotometeric(UV)and liquid chromatographic(HPLC)methods for the determination of natamycin in fermentation solution were studied.The results of UV method show that extraction solvent was methanol spiked with 1 % glacial acetic acid with extraction ratio was 1:9.The 303 nm was wavelength was selected for the absorbance measurements.Log-linear regression equation was Y=0.106 6X-0.006 9 and correlation coefficients(R2)=0.999 5.The percent recovery of the lack-of-fit test of UV method was over 99.931 4 %.The results of two methods were essential correspondence.The relative standard deviation of UV method was 0.17 % opposited to the HPLC method.HPLC was validated the optimum determination method.Detection wavelength:303nm,mobile phase:methanol:water:acetic acid 70:30:2(v/v),flow speed 1.00mL/min with Waters2996 diode-array detection.Log-linear regression equation was Y=12 500X+12 600 and correlation coefficients(R2)=0.999 5.Precision test result:relative standard deviation(RSD)of HPLC was 0.753 %.The detection and quantitation limit was 31 ng as the signal noise ratio was 3.