| 几丁质固定化纤维素酶降解壳聚糖的研究 |
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| 引用本文： | 郑连英,曾嘉,朱江峰.几丁质固定化纤维素酶降解壳聚糖的研究[J].化学反应工程与工艺,2001,17(4):344-348. |
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| 作者姓名： | 郑连英 曾嘉 朱江峰 |
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| 作者单位： | 浙江大学生物工程研究所,浙江杭州,310027 |
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| 基金项目： | 浙江省自然科学基金资助 (编号 2 0 0 0 75 |
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| 摘 要： | 采用天然高分子聚合物几丁质作载体，以戊二醛为交联剂，对纤维素酶进行固定化，研究了戊二醛浓度、pH值、加酶量对纤维素酶固定化的影响，并采用固定化酶反应器对壳聚糖进行降解，再通过超滤膜装置进行分离，经液相凝胶色谱分析，降解产物平均分子量为2900，取得了很好的降解效果。同时对该反应器连续运行，测定该固定化酶的半衰期为60天，且降解性能保持稳定。
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| 关 键 词： | 几丁质 纤维素酶 固定化 降解 壳聚糖 壳低聚糖 固定化酶 |
| 文章编号： | 1001-7631(2001)04-0344-05 |
| 修稿时间： | 2001年2月15日 |
STUDIES ON HYDROLYSIS OF CHITOSAN WITH CHITIN IMMOBILIZED CELLULASE |
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| ZHENG Lian ying,ZENG Jia,ZHU Jiang feng.STUDIES ON HYDROLYSIS OF CHITOSAN WITH CHITIN IMMOBILIZED CELLULASE[J].Chemical Reaction Engineering and Technology,2001,17(4):344-348. |
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| Authors: | ZHENG Lian ying ZENG Jia ZHU Jiang feng |
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| Abstract: | Cellulase was immobilized on chitin by glutaraldehyde through cross link reaction in this work. The effects of glutaraldehyde concentration, pH, and the volumn of enzyme solution on the immobilized enzyme and the optimum conditions were determined. Furthermore, the immobilized enzyme column was used to hydrolyze chitosan, and the product of hydrolysis was separated by a ultrafiltration system. The average molecule weight of the product after separation was 2900 by GPC, which showed excellent hydrolysis ability. The immobilized enzyme bioreactor run stably and effectively, and the remaining of immobilized enzyme activity was 58% after continual work for 60 days. |
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| Keywords: | Chitin Cellulase Immobilization Hydrolyze Chitosan Chitooligosaccharides |
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