Enhancement of G protein-coupled signaling by DHA phospholipids |
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Authors: | Drake?C?Mitchell Shui-Lin?Niu Email author" target="_blank">Burton?J?LitmanEmail author |
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Affiliation: | (1) Laboratory of Membrane Biochemistry and Biophysics, National Institute on Alcohol Abuse and Alcoholism, Park Bldg., Room 158, MSC 8115, 12420 Parklawn Dr., 20892-8115 Bethesda, Maryland |
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Abstract: | The effect of phospholipid acyl chain and cholesterol composition on G protein-coupled signaling was studied in native rod
outer segment (ROS) disk and reconstituted membranes by measuring several steps in the visual transduction pathway. The cholesterol
content of disk membranes was varied from 4 to 38 mol% cholesterol with methyl-β-cyclodextrin. The visual signal transduction
system rhodopsin, G protein (Gt), and phosphodiesterase (PDE)] was reconstituted with membranes containing various levels of phospholipid acyl chain unsaturation,
with and without cholesterol. ROS membranes from rats raised on n−3 fatty acid-deficient and-adequate diets were also studied.
The ability of rhodopsin to form the active metarhodopsin II conformation and bind Gt was diminished by a reduction in the level of DHA (22∶6n−3) acyl chains or an increase in membrane cholesterol. DHA acyl
chain containing phospholipids minimized the inhibitory effects of cholesterol on the rate of rhodopsin-Gt coupling. The activity of PDE, which is a measure of the integrated signal response, was reduced in membranes lacking or
deficient in DHA acyl chains. PDE activity in membranes containing docosapentaenoic acid (DPA, 22∶5n−6) acyl chains, which
replace DHA in n−3 fatty acid deficiency, was 50% lower than in DHA-containing membranes. Our results indicate that efficient
and rapid propagation of G protein-coupled signaling is optimized by DHA phospholipid acyl chains. |
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