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桑叶多糖的分离纯化与分析
引用本文:吕庆,夏玮,张文清,罗国安. 桑叶多糖的分离纯化与分析[J]. 食品与发酵工业, 2007, 33(7): 167-170
作者姓名:吕庆  夏玮  张文清  罗国安
作者单位:1. 华东理工大学分子与工程学院,上海,200237
2. 华东理工大学分子与工程学院,上海,200237;清华大学化学系,北京,100084
摘    要:使用DEAE纤维素和凝胶过滤色谱从桑叶粗多糖中纯化得到了3种多糖组分(SD2-3,SD3-3,SD3-4),经过高效凝胶渗透色谱分析表明,它们为均一多糖,相对分子质量为6000、80000、10000左右。红外光谱分析表明,3种多糖组分SD2-3,SD3-3,SD3-4都可能含有糖醛酸。

关 键 词:桑叶多糖  分离纯化  液相色谱  红外光谱
修稿时间:2007-04-18

The Purification and Analyses of Polysaccharides in Mulberry Leaves
Lv Qing,Xia Wei,Zhang Wenqing,Luo Guoan. The Purification and Analyses of Polysaccharides in Mulberry Leaves[J]. Food and Fermentation Industries, 2007, 33(7): 167-170
Authors:Lv Qing  Xia Wei  Zhang Wenqing  Luo Guoan
Affiliation:1School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237 ,China;2Departmnet of Chemistry, Tsinghua University, Beijing 100084,China
Abstract:Three ingredients(SD2-3,SD3-3,SD3-4) were obtained from crude polysaccharides which were extracted from Mulberry Leaves by gelcolumn chromatography and DEAE-cellulose, their homogeneities were verified by HPGPC, the molecular weight of SD2-3,SD3-3 and SD3-4 were about 6 000,80 000 and 10 000Da. Infrared spectra analysis showed three ingredients (SD2-3 , SD3-3,SD3-4) maybe contained uronic acid.
Keywords:polysaccharide of mulberry leaves  separation and purification   HPLC  infrared spectra
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