| 实时定量PCR法对羊肉中鸡源性成分的量化检测 |
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| 引用本文： | 薛晨玉, 宋丽萍, 路勇, 赵琳娜, 王丹, 杨红莲, 郭淼, 杨昕霆, 胡智恺. 实时定量PCR法对羊肉中鸡源性成分的量化检测[J]. 食品工业科技, 2014, (17): 294-297. DOI: 10.13386/j.issn1002-0306.2014.17.0056 |
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| 作者姓名： | 薛晨玉 宋丽萍 路勇 赵琳娜 王丹 杨红莲 郭淼 杨昕霆 胡智恺 |
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| 作者单位： | 1.北京市食品安全监控中心 |
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| 摘 要： | 为实现羊肉中鸡源性成分的量化检测,本文利用实时荧光PCR技术,通过在单拷贝基因上设计引物,DNA标准曲线绘制以及确定羊肉,鸡肉质量与DNA数学换算参数等方法,对四种不同掺混比例的鸡肉、猪肉混合样本掺混的质量百分比进行分析。结果显示,检测百分比与理论混合百分比间的绝对误差控制在5%以内,量化研究结果准确,该法为食品安全检测工作提供了技术支撑。
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| 关 键 词： | 肉类掺假 量化研究 实时荧光PCR |
| 收稿时间： | 2013-12-25 |
Real-time PCR method for quantitative detect chicken blending in sheep |
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| XUE Chen-yu, SONG Li-ping, LU Yong, ZHAO Lin-na, WANG Dan, YANG Hong-lian, GUO Miao, YANG Xin-ting, HU Zhi-kai. Real-time PCR method for quantitative detect chicken blending in sheep[J]. Science and Technology of Food Industry, 2014, (17): 294-297. DOI: 10.13386/j.issn1002-0306.2014.17.0056 |
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| Authors: | XUE Chen-yu SONG Li-ping LU Yong ZHAO Lin-na WANG Dan YANG Hong-lian GUO Miao YANG Xin-ting HU Zhi-kai |
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| Affiliation: | 1.Beijing Municipal Center for Food Safety Monitoring and Risk Assessment |
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| Abstract: | A real-time PCR method was provided for quantitative analysis the chicken blending in pig.The primers which target genes were single copy in genome were designed.A series of genome DNA were used as standard curve in real-time PCR.The mathematical conversion parameters about the mass of meat and DNA were determined.The weight /weight equivalents of four blending samples were analyzed.The results showed that the absolute error of detection with a tolerance of less than 5%.This research can provide technical support for food safety. |
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| Keywords: | meat adulteration quantitative analysis real-time PCR |
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