柚皮素止咳化痰平喘作用的研究

目的:本实验研究了柚皮素对止咳、排痰、平喘动物模型的治疗作用。方法:采用氨水引咳小鼠模型和枸橼酸引咳豚鼠模型观察柚皮素止咳作用;小鼠酚红排痰模型观察柚皮素化痰作用;磷酸组胺致喘豚鼠模型观察柚皮素平喘作用。结果:柚皮素可以剂量依赖性地延长引起半数小鼠咳嗽的氨水喷雾时间(EDT50);柚皮素低剂组可以显著性地减少枸橼酸引起豚鼠咳嗽次数(p<0.01),柚皮素高剂组可以显著性地延长枸橼酸引起豚鼠咳嗽潜伏期(p<0.01),延长率为165.3%,并减少咳嗽次数(p<0.01)。柚皮素高剂组显著性地增加小鼠气管酚红排量(p<0.05),化痰率为150.9%;柚皮素高剂组可以显著性地延长磷酸组胺诱导豚鼠哮喘出现的潜伏期(p<0.01),延长率为242.2%。结论:柚皮素具有明显的止咳、化痰、平喘作用。 

Experimental study on antitussive,expectorant and anti-asthmatic effects of Naringenin

Objective: The experimental study on the relieving cough, expectoration, relieving asthma effects of naringenin on animal.Method: The cough models induced by ammonia liquor in mice and citric acid in guinea pigs were used to observe the antitussive effects.The methods of phenol red excretion in mice were used to investigate the expectorant effects. Bronchial asthma model induced by histamine phosphate in guinea pigs was used to observe the antiasthmatic effects.Result: The experimental result showed that naringenin could dose dependent to extend ammonia spraying time which could cause half of the mice cough, naringenin low dose group could decrease the frequency of cough inducd by citric acid in guinea pigs ( p < 0.01) , naringenin high dose group could prolong the latent period of cough inducd by citric acid in guinea pigs ( p < 0.01) , extension rate was 165.3%, and decrease the frequency of cough ( p < 0.01) . Naringenin high dose group could also obviously increase the mice tracheal phenol red output ( p < 0.05) ; Sputum excretion rate was 150.9% and naringenin high dose group could obviously prolong the latent period of asthma induced by histamine phosphate in guinea pigs ( p < 0.01) , the extension rate was 242.2%. Conclusion: The naringenin has significant antitussive, expectorant and antiasthmatic effects.