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几种抑菌剂对腊肉原料肉的减菌化效应
引用本文:黄艳杰, 许杨, 张泓. 几种抑菌剂对腊肉原料肉的减菌化效应[J]. 食品工业科技, 2014, (20): 165-169. DOI: 10.13386/j.issn1002-0306.2014.20.027
作者姓名:黄艳杰  许杨  张泓
作者单位:南昌大学食品科学与技术国家重点实验室,中德联合研究院;中国农业科学院农产品加工研究所
摘    要:为了研究几种抑菌剂对腊肉原料肉中腐败菌和致病菌的减菌化效果,选用食用酒精(Edible alcohol,EA)、次氯酸钠(NaClO)、乳酸链球菌素(Nisin)、蛋壳粉末(Eggshell powder,EP)和贝壳碳酸钙(Scallop shell calcium carbonate,SCC)五种抑菌剂对腊肉原料肉进行抑菌实验,研究结果表明抑菌剂的复合使用比单独使用的抑菌效果显著。EA+NaClO+Nisin+EP+SCC组合抑菌效果显著,使菌落总数从5.95lg(cfu·g-1)降至3.35lg(cfu·g-1)(p<0.05)。对大肠杆菌的抑制最显著的是NaClO+Nisin+EP+SCC组合,大肠杆菌数从4.95lg(cfu·g-1)降至2.51lg(cfu·g-1)(p<0.05)。EA+Nisin+EP+SCC组合对单增李斯特菌和酵母菌有很强的减菌化效果,分别从4.53lg(cfu·g-1)和4.20lg(cfu·g-1)降至1.71lg(cfu·g-1)(p<0.05)和1.94lg(cfu·g-1)(p<0.05)。EA+NaClO+EP+SCC组合则具有广谱的抑菌效果,使金黄色葡萄球菌数由4.67lg(cfu·g-1)降至1.74lg(cfu·g-1)(p<0.05),沙门氏菌从4.30lg(cfu·g-1)减少至1.42lg(cfu·g-1)(p<0.05)以及根霉菌从4.13lg(cfu·g-1)降至2.22lg(cfu·g-1)(p<0.05)。因此,和单独使用一种抑菌剂相比,几种抑菌剂复合使用比单独使用抑菌作用显著性增强。 

关 键 词:腊肉原料肉  抑菌剂  腐败菌和致病菌  减菌化
收稿时间:2014-02-11

Synergistic effect of several bacteriostatic agents on bacteria-reduction of raw material for cured meats
HUANG Yan-jie, XU Yang, ZHANG Hong. Synergistic effect of several bacteriostatic agents on bacteria-reduction of raw material for cured meats[J]. Science and Technology of Food Industry, 2014, (20): 165-169. DOI: 10.13386/j.issn1002-0306.2014.20.027
Authors:HUANG Yan-jie  XU Yang  ZHANG Hong
Affiliation:1.State Key Laboratory of Food Science and Technology, Sino-German Joint Research Institute, Nanchang University;2.Institute of Agro-Products Processing Science and Technology, Chinese Academy of Agricultural Science
Abstract:To investigate the effect of several bacteriostatic agents including edible alcohol (EA) , NaClO, Nisin, eggshell powder (EP) and scallop shell calcium carbonate (SCC) on bacteria-reduction of raw material for cured meat. Composite using of the bacteriostatic agents showed a synergistic activity of bacteria-reduction against spoilage as well as pathogenic bacteria. The combination of EA+NaClO+Nisin+EP+SCC made the total number of bacteria colonies decreased from 5.95lg (cfu·g-1) to 3.35lg (cfu·g-1) (p<0.05) . The E. coli colonies were reduced by NaClO+Nisin+EP+SCC from 4.95lg (cfu·g-1) down to 2.51lg (cfu·g-1) (p<0.05) . The optimum bacteria-reduction effect on Listeria monocytogenes as well as Saccharomycetescerevisiae were the combination of EA+Nisin+EP+SCC, decreasing from 4.53lg (cfu·g-1) and 4.20lg (cfu·g-1) to 1.71lg (cfu·g-1) and 1.94lg (cfu·g-1) , respectively (p<0.05) . The combination of EA+NaClO+EP+SCC had broad spectrum effects on bacteria-reductions, which made Staphylococcus aureus colonies decreased from 4.67lg (cfu·g-1) to 1.74lg (cfu·g-1) , Salmonellaenterica colonies decreased from 4.30lg (cfu·g-1) to 1.42lg (cfu·g-1) , and Rhizopusstolonifer colonies decreased from 4.13lg (cfu·g-1) to 2.22lg (cfu·g-1) (p<0.05) . In a conclusion, composite using of antibacterial agent showed a synergistic activity against spoilage and pathogenic bacteria.
Keywords:raw material for cured meat  bacteriostatic agents  spoilage and pathogenic bacteria  bacteria-reduction
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