红曲液态发酵生产橙、黄色素及其色调研究

从大量红曲菌中初选出了10株菌落颜色偏黄的菌株,并对其摇瓶发酵所得到的色素提取液进行了分析,探究了其色素的特性。对色素提取液做了全波长扫描,测定了各发酵液色素的吸收峰所对应的波长和OD值,并计算了色价和色调。通过发酵条件的控制,这10株红曲菌的发酵液中主要色素是黄色素、橙色素或橙黄二者所组成的混合色素,而没有红色素。从中挑选出高产黄色素和橙色素的红曲菌株Monascus sp.961和Monascus sp.W1,其发酵液色价分别为200U/mL(黄色素)和436U/mL(橙色素),并对其色素提取液做了硅胶薄层层析分离,进一步明确了这2株红曲菌所产色素的类型。此研究为红曲黄、橙色素的大规模生产开发及应用奠定基础。 

Study on the production of orange /yellow pigments and their color tone in submerged fermentation of Monascus

In this study, 10 Monascus strains with yellow colony color were primarily screened from a large number of strains for pigments production.Using the method of wavelength scanning, the pigment extracts of the 10 strains by shaking flask fermentation was analyzed and the characteristics of the pigments were explored.The absorption peaks and corresponding OD of pigment extracts were obtained to calculate the color value and tone. By controlling the fermentation conditions, the main pigments in fermented liquid of the 10 Monascus strains were yellow, orange or mixture of yellow and orange, but no red pigment.The Monascus sp.961 with high yellow pigment producing capacity and Monascus sp. W1 with high orange pigment producing capacity were obtained, with the color value up to 200 U /mL and 436 U /mL, respectively.In addition, the types of the pigments produced by the two Monascus stains were further identified according to TLC separation.This study lay the foundation for the production, development and application of Monascus yellow and orange pigments in large-scale fermentation.