响应面法优化复合酶提取香椿叶总膳食纤维的工艺参数

以香椿叶为试材,采用单因素实验和响应面分析法对复合酶(纤维素酶∶淀粉酶=1∶1)提取香椿叶总膳食纤维(TDF)的工艺参数进行了优化。结果表明:在复合酶添加量0.5%,酶解时间2.0h,酶解温度61℃,酶解pH6.1时,香椿叶TDF提取率最高可达74.01%;所得TDF杂质含量低,生理活性好;持水力和膨胀力分别为6.50g/g和4.50mL/g。 

Optimization of process parameters for total dietary fiber from leaves of Toona Sinensis with compound enzyme method by response surface methodology

The method of single factor test and response surface analysis was adopted to optimize the enzymatic extraction of total dietary fiber ( TDF) from leaves of Toona sinensis with cellulose and amylase ( 1∶1) .The results showed that enzyme dosage 0.5%, enzymolysis time 2.0h, temperature 61℃, and pH6.1. The TDF extracting rate was 74.01% under these optimum conditions with low levels of impurities.The physiological activation was good, the holding water capacity and swelling property were 6.50 g /g and 4.50 mL /g respectively.