葡萄籽原花青素对秀丽隐杆线虫抗衰老的影响

以秀丽隐杆线虫作为模型,研究了葡萄籽原花青素(grape seed procyanidins,GSP)对线虫寿命、生殖能力和运动能力的影响,进行了热应激和氧化应激实验,探讨了原花青素影响线虫衰老的方式。结果表明:当原花青素浓度分别为100、200、300μg/mL时,与对照组相比,采用200μg/mL原花青素喂养秀丽隐杆线虫,线虫的平均寿命延长了25.7%(p<0.01),最大寿命延长了24.0%(p<0.01),线虫的运动能力增强,平均每天每分钟爬过的峰数延长了13.2%(p<0.05),可以作为喂养秀丽隐杆线虫的最佳剂量。与对照组相比,采用200μg/mL原花青素饲喂线虫,线虫的产卵高峰期延迟,但对产卵量总数和孵化率无显著影响;热应激胁迫中,线虫的平均生存时间显著延长了27.3%(p<0.01);氧化应激胁迫中,线虫的平均生存时间延长了11.2%(p<0.05)。结论:饲喂葡萄籽原花青素可以改善线虫的平均寿命,且主要是通过提高线虫的压力应激能力来延缓秀丽隐杆线虫的衰老。 

Potential anti-aging effects of grape seed procyanidins on Caenorhabditis elegans

Caenorhabditis elegans was used as a lifespan model to evaluate the anti-aging potential of grape seed procyanidins (GSP) . The tolerance of C. elegans and paraquat-induced oxidization in NGM medium containing GSP (100, 200, 300μg/mL, respectively) were tested. Compared with the controls, feeding of 200μg·mL-1GSP to C. elegans improved significantly the life span of the nematodes. The mean lifespan of C. elegans was lengthened by 25.7% (p <0.01) , and its maximum lifespan increased by 24.0% (p <0.01) . In addition, the nematodes showed a good movement ability, and their average daily number of peaks per minute were prolonged significantly by 13.2% (p<0.05) . Compared to the controls, feeding nematodes of 200μg/mL GSP to C. elegans caused the nematode's fecundity to postpone 1 day, although their total mean fecundity and hatchability had no significant differences from those of the controls. In the presence of 20μg/mL GSP, the mean survival time of C. elegans was lifted by 27.3% for thermo-tolerance (p<0.01) and 11.2% (p<0.05) for paraquat-induced oxidization, respectively. Apparently, consumption of grape seed procyanidins prolonged the average lifespan of the nematodes mainly because C. elegans improved its tolerance towards stress conditions which caused aging.