| Lipozyme TL 100L脂肪酶的固定化及其性质研究 |
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| 引用本文： | 朱珊珊,邵佩霞,王永华.Lipozyme TL 100L脂肪酶的固定化及其性质研究[J].食品工业科技,2010(5). |
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| 作者姓名： | 朱珊珊 邵佩霞 王永华 |
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| 作者单位： | 华南理工大学轻工与食品学院,广东,广州,510640 |
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| 摘 要： | 比较了8种大孔树脂对脂肪酶Lipozyme TL 100L的固定化效果,选取树脂AB-8为该酶最佳的固定化载体,并对固定化条件进行了优化,得到AB-8固定化脂肪酶的最佳pH为9.0,最佳吸附量为54.5mg/g树脂,最佳含水量为6%～7%。固定化脂肪酶的pH稳定性、热稳定性、操作稳定性和贮存稳定性均有一定程度的提高。
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| 关 键 词： | 脂肪酶 固定化 大孔树脂 优化 稳定性 |
Study on immobilization of Lipase TL 100L and its properties |
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| ZHU Shan-shan,SHAO Pei-xia,WANG Yong-hua.Study on immobilization of Lipase TL 100L and its properties[J].Science and Technology of Food Industry,2010(5). |
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| Authors: | ZHU Shan-shan SHAO Pei-xia WANG Yong-hua |
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| Affiliation: | ZHU Shan-shan,SHAO Pei-xia,WANG Yong-hua(College of Light Industry , Food Science,South China University of Technology,Guangzhou 510640,China) |
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| Abstract: | Eight kinds of macroporous resin were used on immobilizing lipase Lipozyme TL 100L.Resin AB-8 was the best carrier,and the immobilization conditions were optimized. The best immobilization pH was 9.0,the best adsorption capacity was 54.5mg/g resin,and the best water content was 6% to 7%. The pH stability,thermal stability,operational stability and storage stability of immobilized lipase were all improved. |
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| Keywords: | lipase immobilization macroporous resin optimization stability |
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