中华蹄盖蕨抗番茄溃疡病菌活性物质提取工艺优化及其抑菌作用研究

本研究以获得抗番茄溃疡病菌的植物源活性物质为目的。在单因素实验基础上以抑菌活性为指标采用有交互作用的正交试验设计L₂₇(3¹³),确定中华蹄盖蕨抗番茄溃疡病菌活性物质的提取工艺。将所得活性物质作用于番茄溃疡病菌,测定菌体内活性氧、细胞内蛋白含量、细胞外蛋白含量的变化。结果表明,料液比和提取温度对活性物质的抑菌效应有极显著影响,且它们之间的交互作用有显著影响。确定最优提取条件为料液比1:10 g/mL、提取温度80℃、提取时间8 h,其抑菌圈直径为27.33 mm。中华蹄盖蕨活性物质处理番茄溃疡病菌后,胞内活性氧水平明显升高,细胞外蛋白含量增加,细胞内蛋白含量降低。以上研究可得,以中华蹄盖蕨为原料可获得有效抗番茄溃疡病菌的活性物质,为该病菌的防治提供新的途径。

Optimization of Extraction Process and Antibacterial Activity of Anti-Clavibacter michiganensis subsp.michiganensis of Active Substances from Athyrium sinense

The aim of this study was to obtain effective substances from plant against Clavibacter michiganensis subsp. michiganensis. On the basis of single factor experiments,an interactive orthogonal experiment L₂₇(313)was designed. Antibacterial activity was used to optimize the extraction conditions of active substances from Athyrium sinense against C. michiganensis subsp.michiganensis. The active substances obtained under the optimal conditions were used to determine the three indexes of active oxygen,intracellular protein contents and extracellular protein contents in C. michiganensis subsp. michiganensis. The results showed that material to liquid ratio and temperature had extremely significant effects on the extraction of antibacterial active substances,and the interaction between them had a significant effect. The optimal extraction conditions were determined as material to liquid ratio 1:10 g/mL,extraction temperature 80 ℃ and extraction time 8 h,and the diameter of the inhibition zone was 27.33 mm. After treatment with A. sinense active substance,the levels of intracellular reactive oxygen species and extracellular protein content increased,and the intracellular protein content decreased. According to the results,the active substance of A. sinense against C. michiganensis subsp.michiganensis can be obtained,which should provide a new idea for the control of this pathogen.