Quantification of human immunodeficiency virus type 1 RNA levels in plasma by using small-volume-format branched-DNA assays |
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Authors: | T Yeghiazarian Y Zhao SE Read W Kabat X Li SJ Hamren PJ Sheridan JC Wilber DN Chernoff R Yogev |
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Affiliation: | Chiron Diagnostics, Emeryville, California 94608-2916, USA. tory_yeghiazarian@cc.chiron.com |
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Abstract: | We have developed small-volume (50 or 250 microl)-format branched-DNA assays for human immunodeficiency virus type 1 (HIV-1) RNA for use with specimens in which the volume is limited and/or a high viral load is anticipated. These formats exhibited good correlation with the standard 1-ml format; high specificity, reproducibility, and linearity; and no significant difference in the quantification of HIV-1 subtypes. |
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