不同补料方式对酿酒酵母高密度发酵的影响

以酿酒酵母为发酵菌种，使用自主优化筛选配制的糖蜜培养基，分别采用残糖反馈分批补料和连续流加补料方式，优化发酵工艺条件以提高发酵液中菌体密度。残糖反馈分批补料，拟设定3个梯度，分别控制对数期发酵液中残糖含量在0.5%～1.5%，1.0%～2.0%，1.5%～2.5%。三次试验结果分别为，试验1菌体培养32 h时达到最大生物量，为28.88 g/L；试验2菌体培养52 h时达到最大生物量，为27.43 g/L；试验3菌体培养24 h时达到最大生物量，为22.06 g/L。通过连续流加补料培养菌体，结果培养30 h时达到最大生物量，为29.29 g/L。

Effect of fed-batch fermentation modes on high density fermentation of Saccharomyces cerevisiae

Using Saccharomyces cerevisiae as fermentation starter, the fermentation process for improving S. cerevisiae density in the laboratory screened single molasses medium was optimized by residual glucose feedback fed-batch and continuous flow plus feeding mode. The fed-batch of glucose feedback test was set three gradients to control the content of residual glucose (soluble glucose) in the fermentation broth of 0.5%-1.5%, 1.0%- 2.0%, 2.0%-2.5%, respectively. The results showed that yeast cells in test 1 fermentation broth(0.5%-1.5%) reached the maximum biomass concentration (MBC) with dry cell weight (DCW) of 28.88 g/L at 32 h; yeast cells in test 2 (1.0%-2.0%) fermentation broth reached the MBC with DCW of 27.43 g/L in 52 h; and yeast cells in test 3 (1.5%-2.5%) fermentation broth reached the MBC with DCW of 22.06 g/L in 24 h. The feeding test results with continuous flow showed that yeast cells in the fermentation broth reached the MBC (DCW=29.29 g/L) in 30 h.