Advances in Quantitative FRET‐Based Methods for Studying Nucleic Acids |
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Authors: | Søren Preus Assoc. Prof. L. Marcus Wilhelmsson |
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Affiliation: | 1. Department of Chemistry, University of Copenhagen, Universitetsparken 5, 2100 Copenhagen (Denmark);2. Department of Chemical and Biological Engineering, Chalmers University of Technology, 41296 G?teborg (Sweden) |
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Abstract: | Förster resonance energy transfer (FRET) is a powerful tool for monitoring molecular distances and interactions at the nanoscale level. The strong dependence of transfer efficiency on probe separation makes FRET perfectly suited for “on/off” experiments. To use FRET to obtain quantitative distances and three‐dimensional structures, however, is more challenging. This review summarises recent studies and technological advances that have improved FRET as a quantitative molecular ruler in nucleic acid systems, both at the ensemble and at the single‐molecule levels. |
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Keywords: | DNA structures fluorescence spectroscopy fluorescent probes FRET nucleic acids |
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