An Arduino based automatic pressure evaluation system to quantify growth of non-model anaerobes in culture |
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Authors: | St Elmo Wilken Patrick A Leggieri Corey Kerdman-Andrade Matthew Reilly Michael K Theodorou Michelle A O'Malley |
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Affiliation: | 1. Department of Chemical Engineering, University of California, Santa Barbara, California, USA;2. Department of Biology, University of York, York, UK;3. Agriculture Centre for Sustainable Energy Systems, Animal Production, Welfare and Veterinary Sciences, Harper Adams University, Newport, UK |
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Abstract: | Measuring the growth rate of non-model anaerobic microbes typically requires the use of time-consuming and often destructive manual measurements. Here, an Arduino based automatic pressure evaluation system (A-APES) was developed to automatically measure the rate of fermentation gas production as a proxy for microbial growth in anaerobic systems. The A-APES system measures accumulated gas pressure in sealed cultures accurately at high-resolution, while venting the system at programmed intervals to prevent over pressurization. The utility of A-APES is demonstrated in this study by quantifying the growth rate and phases of a biomass-degrading anaerobic gut fungus, which cannot be otherwise measured via conventional techniques due to its association with particulate substrates. Given the utility of the A-APES approach, we provide a complete construction guide to fabricate the device, which is three times less expensive compared to existing commercial alternatives. |
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Keywords: | anaerobic fungi Arduino automatic culture equipment non-model microbe pressure transducer |
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