Quantitative Polarization-Resolved Second-Harmonic-Generation Microscopy of Glycine Microneedles |
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Authors: | Matthew Gleeson Kevin O'Dwyer Sarah Guerin Daragh Rice Damien Thompson Syed A M Tofail Christophe Silien Ning Liu |
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Affiliation: | Department of Physics and Bernal Institute, University of Limerick, Castletroy, Co. Limerick, Limerick, V94 T9PX Ireland |
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Abstract: | Second-harmonic generation (SHG) is a nonlinear optical process that can provide disease diagnosis through characterization of biological building blocks such as amino acids, peptides, and proteins. The second-order nonlinear susceptibility tensor χ(2) of a material characterizes its tendency to cause SHG. Here, a method for finding the χ(2) elements from polarization-resolved SHG microscopy in transmission mode is presented. The quantitative framework and analytical approach that corrects for micrometer-scale morphology and birefringence enable the determination and comparison of the SHG susceptibility tensors of β- and γ-phase glycine microneedles. The maximum nonlinear susceptibility coefficients are d33 = 15 pm V−1 for the β and d33 = 5.9 pm V−1 for the γ phase. The results demonstrate glycine as a useful biocompatible nonlinear material. This combination of the analytical model and polarization-resolved SHG transmission microscopy is broadly applicable for quantitative SHG material characterization and diagnostic imaging. |
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Keywords: | biomolecules biophotonics molecular crystal allomorphs |
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