气相色谱法测定海豹油中EPA、DPA、DHA含量的方法学研究

目的：建立气相色谱检测海豹油中EPA、DPA和DHA的方法。方法：采用氢氧化钾-甲醇酯化法将海豹油中的脂肪酸快速、有效的转化成脂肪酸甲酯，并通过方法学实验验证其可行性。结果：EPA、DPA和DHA甲酯含量在10～2000μg/mL范围内，峰面积与EPA、DPA和DHA甲酯含量呈良好线性关系；加样回收率在96.93%～103.38%之间，相对标准偏差小于2%；结论：该方法可以快速、准确地同时测定EPA、DPA和DHA含量。

Determination of EPA,DPA and DHA in Seal Oil by Gas Chromatography

Objective: To establish a method for determining ω-3 polyunsaturated fatty acids including eicosapentaenoic acid (EPA), docosapentenoic acid (DPA) and docosahexaenoic acid (DHA) in seal oil. Methods: Fatty acids in seal oil were methyl esterified using a KOH-methanol solution. The applicability of the developed method was validated. Results: This method revealed an excellent liner relationship for methylated EPA, DPA and DHA in the concentration range of 10－2000μg/mL with recovery rate of 96.93%－103.38% and RSD less than 2%. Conclusion: This method is simple, rapid, accurate and suitable for the analysis of fatty acids in seal oil.