| 原位形成的金纳米颗粒对溶菌酶结构的影响 |
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| 引用本文： | 杨涛,李敏,沈福刚,张洪波,任淑霞,杨超. 原位形成的金纳米颗粒对溶菌酶结构的影响[J]. 微纳电子技术, 2012, 0(8): 516-521 |
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| 作者姓名： | 杨涛 李敏 沈福刚 张洪波 任淑霞 杨超 |
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| 作者单位： | 河北大学化学与环境科学学院;河北大学电子信息工程学院;河北大学质量技术监督学院 |
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| 基金项目： | 河北省自然科学基金资助项目(B2011201107);保定市科学研究与发展计划(11ZF122,11ZG019,11ZG020);河北大学引进人才科研启动基金资助项目(2009-172) |
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| 摘 要： | 在溶菌酶水溶液中,用NaBH4原位还原HAuCl4得到了溶菌酶稳定的金纳米颗粒。这些溶菌酶稳定的金纳米颗粒具有亲水性、生物兼容性以及良好的溶胶稳定性。紫外-可见光谱、透射电子显微镜以及动态光散射实验对所得产物的表征结果说明实验中形成了分散性良好的直径约2 nm的金纳米颗粒。傅里叶变换红外光谱和圆二色谱实验表明,与金纳米颗粒作用之后的溶菌酶分子仍然保持了结构的完整性,金纳米颗粒的形成没有对蛋白质的一级和二级结构造成影响。基于其良好的溶胶稳定性及生物兼容性,这些溶菌酶稳定的金纳米颗粒在纳米科技和生物医学领域具有潜在的应用价值。
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| 关 键 词： | 金纳米颗粒 原位形成 溶菌酶 蛋白质结构 生物兼容性 |
Influence of In-Situ Formed Gold Nanoparticles on the Structure of the Lysozyme |
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| Yang Taoa,Li Minb,Shen Fuganga,Zhang Hongboc,Ren Shuxiaa,Yang Chao. Influence of In-Situ Formed Gold Nanoparticles on the Structure of the Lysozyme[J]. Micronanoelectronic Technology, 2012, 0(8): 516-521 |
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| Authors: | Yang Taoa Li Minb Shen Fuganga Zhang Hongboc Ren Shuxiaa Yang Chao |
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| Affiliation: | a (a.College of Chemistry and Environmental Science;b.College of Electronic and Informational Engineering; c.College of Quality and Technical Supervision,Hebei University,Baoding 071002,China) |
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| Abstract: | Lysozyme-stabilized gold nanoparticles with hydrophilicity,biocompatibility and very good sol stability were synthesized by the chemical reduction of HAuCl4 with NaBH4 in the lysozyme aqueous solution.The UV-VIS spectra,transmission electron microscopy,and dynamic light scattering experiment characterization results of the as-prepared nanoparticles reveal that well dispersed gold nanoparticles are formed with the diameter of 2 nm.The Fourier transform infrared spectroscopy and circular dichroism characterization indicate that the protein molecules have the intactness structure after the interaction with gold nanoparticles,the formation of the gold nanoparticles has no effect on the first and second structures of the protein.Based on their excellent colloidal stability and biocompatibility,the lysozyme-stabilized gold nanoparticles are promising in nanoscience and biomedical applications. |
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| Keywords: | gold nanoparticle in-situ formation lysozyme protein structure biocompatibility |
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