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Sarcoplasmic Reticulum Ca2+-ATPase and Cytochrome Oxidase as Indicators of Frozen Storage in Cod (Gadus morhua)
Authors:H. Godiksen    G. Hyldig    R Jessen
Affiliation:Authors are with the Danish Inst. for Fisheries Research, Dept. of Seafood Research, Technical Univ. of Denmark, Building 221, DK-2800 Lyngby, Denmark. Direct inquiries to author Godiksen (E-mail: ).
Abstract:ABSTRACT: Activities of 2 membrane-bound enzymes, Ca2+-ATPase from the sarcoplasmic reticulum and cytochrome oxidase from the inner mitochondria membrane, were measured during frozen storage of cod. Enzyme activities were higher in cod muscle samples frozen at −30°C than at −20°C. Freezing-induced activation of both enzymes was observed and the activation was amplified by ice storage prior to freezing. Sensory evaluation conducted at 9 mo of frozen storage showed differences between the sensory properties of cod frozen immediately after catch and frozen after 3 d of storage on ice. These results indicated that the enzymes might be useful as indicators of quality changes by frozen storage.
Keywords:cod    cytochrome oxidase    frozen storage    ice storage    SR Ca2+-ATPase
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