高效液相色谱法对白酒中甲醛含量的测定研究

甲醛本身的分子结构简单,不能够直接被反相色谱柱吸附,需要对其进行柱前衍生.本法采用2,4-二硝基苯胼作为衍生液对甲醛进行柱前衍生,生成的2,4-二硝基苯腙被反相色谱柱吸附,用水-乙腈混合溶液洗脱后,采用紫外检测器检测,以保留时间定性,峰面积定量.

Determination of Formaldehyde Content in Liquor With High Performance Liquid Chromatographic Method

Formaldehyde has simple molecular structure,so it can not directly be adsorbed by reversed phase chromatographic column,and need be carried out the precolumn derivatization.In this paper,using 2,4-dinitrophenylhydrazine as a reactant,the precolumn derivatization of formaldehyde was carried out to generate 2,4-dinitrophenylhydrozone,it was adsorbed by reversed phase chromatographic column.Acetonitrile-water mixture was used to elute,and UV detector was used to determine through using retention time as the qualitative index,peak area as the quantitative index.