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全基因组关联分析馒头比容与质构SNP标记
引用本文:刘娟,陈广凤,田纪春,吴澎,赵子彤,杨艺,李向阳,唐晓珍.全基因组关联分析馒头比容与质构SNP标记[J].中国粮油学报,2018,33(5):106.
作者姓名:刘娟  陈广凤  田纪春  吴澎  赵子彤  杨艺  李向阳  唐晓珍
作者单位:山东省高校食品加工技术与质量控制重点实验室山东农业大学食品学院;德州学院生态与景观学院;山东农业大学小麦品质育种研究室山东省作物生物学重点实验室
基金项目:山东省重点研发计划(公益类) “彩麦精深加工技术和新产品研发” 2017GNC10101; 山东农业大学作物生物学国家重点实验室开放课题(2015KF14)
摘    要:为从分子水平研究控制馒头比容与质构性状的基因位点,以205份不同小麦品种为实验材料,利用分布于小麦全基因组的24 355个单核苷酸多态性(SNP)标记对馒头比容与质构性状进行关联分析。共检测到42个比容性状显著关联位点,其中8个极显著关联位点(P0.000 1),同时也是高遗传贡献率位点(R~210%),2个位点至少在2个环境中稳定表达;检测到313个质构性状显著关联位点,其中31个极显著关联位点,46个高遗传贡献率位点,11个位点至少在2个环境中稳定表达。同时,发掘了5个质构性状主效关联位点,如3B染色体上黏聚性关联位点Kukri_c13329_800等。本研究所得到的这些标记为在分子水平上研究馒头品质性状提供了有价值的参考。

关 键 词:馒头比容  馒头质构  单核苷酸多态性标记  全基因组关联分析  显著关联位点
收稿时间:2017/10/9 0:00:00
修稿时间:2017/12/15 0:00:00

Genome-Wide Association Analysis between SNP Markers and Specific volume and Texture Related Traits of Steamed Bread
Abstract:In order to study the gene loci that control specific volume and texture of steamed bread, 205 diverse wheat varieties were used to conduct trait-markers association using 24355 single nucleotide polymorphism (SNP) markers covered the whole genome of wheat. In the results, 42 significiant markers were detected for specific volume of steamed bread distributed across 7 chromosomes of wheat, including 8 highly significiant markers (P<0.0001), as well as high genetic contribution markers (R2>10%), 2 markers detected in two or more environment. 313 significiant markers were detected for texture traits of steamed bread mapped onto 15 chromosomes of wheat, including 31 highly associated markers(P<0.0001), 46 high genetic contribution markers and 11 stable markers. At the same time,5 main sites were detected for texture of steamed bread, such as Kukri_c13329_800 on chromosome 3B. These results will facilitate further researches in sensory properties in steamed bread.
Keywords:bread  specific volume  bread  texture  single  nucleotide polymorphism  markers  genome-wide  association analysis  significiant  markers
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