壳聚糖修饰植物甾醇脂质体的制备及稳定性研究

采用乙醇注入法制备植物甾醇脂质体(Phytosterol Liposome,PLs),并以不同浓度壳聚糖进行修饰优化制备工艺;通过粒径、Pd I、电位和稳定性指数,分析评价了壳聚糖修饰植物甾醇脂质体(Chitosan modified Phytosterol Liposome,CS-PLs)在不同环境下的稳定性;并对壳聚糖修饰前后PLs进行了体外胃肠消化环境稳定性实验。结果表明:当壳聚糖浓度为0.3 mg/mL时可获得粒径小、分布均一的CS-PLs;且pH、温度和离子强度及种类均对CS-PLs稳定性有显著影响;PLs经壳聚糖修饰前后,胃消化稳定性均良好,但在模拟肠消化环境中,经壳聚糖修饰后的PLs表现出更好的稳定性。

Preparation and stability properties of chitosan modified phytosterol liposomes

Phytosterol liposomes (PLs) were prepared by ethanol injection method, and the preparation process was optimized by chitosan modified with different concentration. The stability of chitosan modified phytosterol liposomes (CS-PLs) was analyzed and evaluated by particle size, PdI, Zeta potential and TSI in different environments. It also compared the stability of PLs with CS-PLs in vitro gastrointestinal digestion environment. The results indicated that the small and uniform particle size of CS-PLs can be obtained with chitosan concentration of 0.3mg/mL. The pH, temperature, ionic strength and type have great effect on the stability of CS-PLs. The PLs with or without chitosan modified were stable in gastric digestion; but CS-PLs showed better stability in simulated intestinal digestion than PLs.