4-羟苯维胺酯抑制大鼠膀胱癌的COX-2的表达和诱导肿瘤细胞凋亡的实验研究

目的 使用N-甲基-N-亚硝基脲(N-methyl-N-nitrosourea,MNU)诱导SD大鼠膀胱肿瘤模型,观察4-羟苯维胺酯(4-HPR)的对大鼠膀胱肿瘤的干预效果。方法 70只SD大鼠随机分成对照组(A组)、造模组(B组)、造模处理组(C组、D组、E组),处理组分别予以4-HPR、阿霉素(ADM)、卡介苗(BCG)行膀胱内灌注。给药结束后1周处死大鼠,用免疫组化检测大鼠膀胱肿瘤中COX-2的表达和微血管密度(MVD) ;用TUNEL法检测凋亡。结果 标本病理结果提示4-HPR、BCG、ADM对MNU诱导肿瘤的发生有明确的干预效果(P<0.05)。4-HPR能有效抑制COX-2的表达及微血管的生成并促使肿瘤细胞的凋亡(P<0.05)。结论 4-HPR、BCG、ADM对MNU的致癌过程均有干预作用,4-HPR与ADM、BCG的抗癌作用无显著性差异,且其副反应更少。

Prevention effect of 4-HPR on bladder tumor in SD rats: An in vivo experiment

AIM: To observe the curative effect of 4-HPR on bladder tumor using bladder tumor model induced by MNU in SD rats. METHODS: 70 SD rats was randomly divided into 5 groups: control group (Group A), module group (Group B), mould processing group (Group C, Group D, Group E). The bladder perfusion of treatment group was instilled 4-HPR, ADM and BCG respectively. A week after administration, the SD rats were put to death. The immunohistochemistry was used to detecte the expression of COX-2 and MVD, and the TUNEL technique was applied to examine apoptosis.RESULTS: The results of pathological specimens indicated that the 4-HPR, ADM and BCG inducing tumor had an obvious intervention effect (P<0.05); Besides, the 4-HPR could inhibit the expression of COX-2, the generation of capillaries, and the spur of apoptosis tumor cells (P<0.05).CONCLUSION: The 4-HPR, ADM and BCG to MNU had an intervention effect in carcinogenic process. The 4-HPR had no significant differences in anticancer effect compared with other groups, but had less side effect than the other groups.