当归挥发油对大鼠局灶性脑缺血再灌注损伤的保护作用

目的: 探讨当归挥发油对大鼠局灶性脑缺血再灌注损伤的保护作用。方法: 采用改良线栓法阻断大鼠大脑中动脉建立脑缺血模型,到达再灌注时限后进行神经功能缺损评分;检测脑梗死体积比、脑含水量及脑血管通透性;血清NO、NOS含量;以及SOD和GSH-Px活性。结果: 当归挥发油可有效改善脑缺血再灌注损伤大鼠的神经功能缺损;降低脑梗死体积比、脑血管通透性和脑含水量;降低血清 NO、NOS含量; 增强SOD和GSH-Px活性。结论: 当归挥发油对脑缺血再灌注损伤有显著保护作用,其机制可能与提高脑组织抗氧化能力有关。

Protection of volatile oil from Angelica sinensis on focal cerebral ischemia-reperfusion in rats

AIM: To discuss the protection of volatile oil from Angelica sinensis on focal cerebral ischemia-reperfusion in rats. METHODS: Focal cerebral ischemia-reperfusion injury model was made with suture-occluded method.The neurological deficits of rats were observed after occluding middle cerebral artery for 45 min and reperfusion for 2 hours.The volume of cerebral infarction were measured; the levels of NOS and NO, and the activities of SOD and GSH-Px in serum were detected. RESULTS: Volatile oil from Angelica sinensis could effectively ameliorate the changes induced by cerebral ischemia-reperfusion, including: meliorate the neurological deficits; decrease the levels of NOS and NO and the volume of cerebral infarction; increase the activities of SOD and GSH-Px. CONCLUSION: Volatile oil from Angelica sinensis shows the protection against ischemia-reperfusion injury, and the effect may related to increase antioxidation of the brain tissue.