中国肾移植患者钙蛋白酶10基因多态性与移植后糖尿病的相关性研究

目的: 探讨中国肾移植患者钙蛋白酶10(CAPN10)基因多态性与移植后糖尿病(PTDM)的相关性。方法: 采用等位基因特异性PCR、限制性片断长度多态性PCR(PCR-RFLP)分别检测了97例PTDM患者(PTDM组)和301例未发生PTDM的肾移植患者(对照组)的CAPN10基因SNP-19、SNP-43、SNP-63的基因型,采用 logistic 回归分析该基因多态性与PTDM的相关性。结果: PTDM组患者SNP-19的11 +12基因型频率和SNP-43的GG基因型频率明显高于对照组(P< 0.05)。用性别、移植时年龄、体重和BMI进行校正后, SNP-19 的11基因型和12基因型携带者移植术后发生PTDM的风险分别是22基因型的1.502倍(OR=1.502,95%CI:1.016~2.347,P=0.048)和1.764倍(OR=1.764,95%CI:1.055~2.947,P=0.030),SNP-43的GG基因型携带者移植术后发生PTDM的风险是AA和GA基因型患者的2.19倍(OR＝2.190,95%CI:1.047~3.473,P=0.044),SNP-63与PTDM的发生无明显相关性(P>0.05)。结论: CAPN10基因SNP-19 的1等位基因和SNP-43的GG基因型是肾移植后发生PTDM的独立危险因素。

Calpain10 gene polymorphisms are associated with posttransplantation diabetes mellitus in Chinese renal allograft recipients

AIM: To investigate the association between the Calpain10 gene polymorphism and the risk of PTDM in Chinese renal allograft recipients.METHODS: Three single nucleotide polymorphisms (CAPN10 gene SNP-19,SNP-43,SNP-63) were genotyped in the cohort, which consisted of 97 renal allograft recipients with PTDM (PTDM group) and 301 renal allograft recipients without PTDM (control group).The genotypes of polymorphisms were performed by allele specific polymerase chain reaction (ASPCR), PCR-restriction fragment length polymorphism (PCR-RFLP). Logistic regression test was used to identify risk factors for PTDM development and calculate the odds ratio.RESULTS: The 11 and 12 genotypes of SNP-19 and the GG genotypes of SNP-43 were more common in patients with PTDM than those without PTDM (P<0.05). After adjustments for age, sex, body weight and BMI, the effect of genotype remained significant (11 vs 22, OR=1.502, 95%CI: 1.016-2.347, P=0.048; 12 vs 22, OR=1.764, 95%CI: 1.055-2.947, P=0.030) in SNP-19 and the patients carrying genotype GG had higher risk comparison with carriers with genotype AA or GA(OR＝2.190, 95%CI: 1.047-3.473, P=0.044)in SNP-43. But SNP-63 was not association with PTDM.CONCLUSION: The 1-allele in SNP-19 and GG genotype in SNP-43 of CAPN10 gene are the independent risk factors of PTDM in Chinese renal allograft recipients.