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Kiyoteru Tokuyasu: a pioneer of cryo‐ultramicrotomy |
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| Authors: | GARETH GRIFFITHS JAN‐WILLEM SLOT PAUL WEBSTER |
| Affiliation: | 1. Department of Biosciences, University of Oslo, Oslo, Norway;2. Department of Cell Biology, University Medical Center Utrecht, Utrecht, The Netherlands;3. Oak Crest Institute of Science, Monrovia, California, U.S.A. |
| Abstract: | In July 2015 Professor K.T. Tokuyasu passed away in San Diego giving us the opportunity to reflect on the contribution this electron microscopist made to the field of immunocytochemistry. His work provided a sensitive, minimally invasive approach to producing thin sections of biological material for labeling with antibodies. His approach has been applied to a wide range of biological applications and provided important information on cellular processes. |
| Keywords: | Cryoultramicrotomy immunocytochemistry thawed cryosections sectioning cryo‐EM vitrification cryo‐protection methyl cellulose Tokuyasu method colloidal gold ferritin CEMOVIS |