碳氮比对Phaffia rhodozyma CS0217 虾青素合成的影响

碳氮比(C/N)对Phaffia rhodozyma CS0217 虾青素的合成有重要影响，摇瓶实验结果表明：随着C/N 的降低，虾青素含量显著降低，当C/N 为4.5:0.1 时，每克干细胞虾青素的最高含量为2.48mg/g；在高C/N 条件下酵母发芽率低，控制酵母发芽率小于0.5，有利于虾青素的合成；进一步通过添加放线菌酮发现，由β - 胡萝卜素向虾青素转化的关键酶可能在细胞培养的对数生长期受到抑制。

Effect of C/N Ratio on Astaxanthin Synthesis in Yeast Phaffia rhodozyma CS0217

Astaxanthin synthesis in yeast Phaffia rhodozyma CS0217 was greatly dependent on C/N ratio.The amount of synthesized astaxanthin exhibited a dramatic decrease due to the decrease of C/N ratio.The highest astaxanthin productivity was 2.48 mg/g at the C/N ratio of 4.5:0.1.High C/N ratio could result in a low budding rate of yeast,which was benefit for astaxanthin synthesis.The optimal budding rate of yeast should be controlled below 0.5.Furthermore,the addition of cycloheximide in the culture medium for yeast...