Characteristic Oxidation Products of Choline Plasmalogens are Detectable in Cattle and Roe Deer Spermatozoa by MALDI-TOF Mass Spectrometry |
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Authors: | Beate Fuchs Karin Müller Frank Göritz Steffen Blottner Jürgen Schiller |
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Affiliation: | 1. Faculty of Medicine, Institute of Medical Physics and Biophysics, University of Leipzig, H?rtelstr. 16-18, 04107, Leipzig, Germany 2. Leibniz Institute for Zoo and Wildlife Research, Berlin, Germany 3. Institute for Reproduction of Farm Animals, Schoenow, Germany
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Abstract: | Plasmalogens (1-O-alk-1′-enyl-2-acyl-sn-glycero-3-phosphocholines and -phosphoethanolamines) are important constituents of spermatozoa membranes and possess significant
antioxidative properties. This particularly holds as plasmalogens from spermatozoa also possess a very high content of highly
unsaturated fatty acyl residues (especially 22:6). The organic spermatozoa extracts of two different ruminants (cattle and
roe deer) were analyzed for their contents of characteristic choline plasmalogen oxidation products by matrix-assisted laser
desorption and ionization time-of-flight (MALDI-TOF) mass spectrometry. It will be shown that 1-hydroxy-2-docosahexaenoyl-sn-glycero-3-phosphocholine (LPC 22:6) and formyl-LPC 22:6 are reliable measures of lipid oxidation of spermatozoa and allow,
accordingly, conclusions about the storage conditions. All data on spermatozoa were also confirmed by the investigation of
the oxidation behavior of selected reference compounds. It will be shown that, equally if plasmalogens or diacyl PC species
are used, oxidation takes place primarily at the double bond next to the glycerol backbone. These data were additionally confirmed
by recording the corresponding post source decay (PSD) fragment ion spectra. |
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Keywords: | Spermatozoa Plasmalogen Lipid oxidation MALDI-TOF MS Post source decay |
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